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1.
Acta odontol. latinoam ; 5(1): 63-9, 1990.
Article in Spanish | LILACS-Express | LILACS, BINACIS | ID: biblio-1157665

ABSTRACT

The existence of sexual dimorphism (SDM) in tubules and acini of the submaxillary gland was investigated in male and female 60 day-old Wistar rats. Conventional histology was followed by evaluation of 1) Percentage of area occupied by tubules, acini, and other tissues and 2) Tubular area, employing a semi-automatic image analyser. The granular tubules of male rats were larger than those of females. This statistically significant difference indicates the existence of SDM from a morphological viewpoint. Castration seems to abolish these differences by inducing involution of tubular tissue in males. Whereas treatment with testosterone seems to reconstitute tubular tissue in castrated males, a similar dose of estradiol benzoate elicits a reduction in tubular size in ovariectomized females.

2.
Rev. Fac. Odontol. (Córdoba) ; 17(1-2): 55-69, 1989 Jan-Dec.
Article in Spanish | LILACS-Express | LILACS, BINACIS | ID: biblio-1171009

ABSTRACT

The object of this work was to establish whether induced stress leads to morphological changes in the sublingual and submaxillary glands of rats. General adaptation syndrome (S.G.A.) was induced in 25 young adult male Wistar rats through cold and immobilization. The animals were killed 48 hrs. and 10 days after stressor (E.A.) application. To verify stress condition, the adrenales were weighed and plasmatic corticosterone were carried out. The sublingual and submaxillary glands were dissected, fixed in buffered formol and stained with H.E. The results histology indicate that the submaxillary is more sensitive to cold than the sublingual, while the reverse holds true form immobilization.

3.
Rev. Fac. Odontol. (Córdoba) ; 17(1-2): 43-53, 1989 Jan-Dec.
Article in Spanish | LILACS-Express | LILACS, BINACIS | ID: biblio-1171010

ABSTRACT

Salivary glands are dependent on sexual hormones. The aim of the present work is to study the behavior of inflammatory response induced in animals that were castrated and injected with estrogens. Male adult wistar castrated rats (60-90 days) were used. A phlogogen pellet (zinc-oxide-turpentine essence) was placed between their sublingual and submaxillary glands and they were daily injected with 5 units of estrogen. The rats were killed after 8 and 12 days of treatment; submandibular pack was weighed, dissected and fixed in phormol Ph7 for its morphohistochemical study. Phlogogen pellet breaks out an acute inflammatory response that appears attenuated in castrated animals. Such character is enhanced when estrogens are used, disappearing ductal ectasis, becoming evident a granulation tissue of strange body in phagocytic activity and in contact with the pellet. As a consequence its follows that estrogen administration in castrated animals attenuates acute inflammatory response broken out by phlogogen pellet, determining characters with tendency to chronicity and giant cells differentiation of strange body.

4.
Rev. Fac. Odontol. (Córdoba) ; 16(1/2): 37-46, ene.-dic. 1988. ilus, tab
Article in Spanish | LILACS | ID: lil-80230

ABSTRACT

La población mastocitaria de glándulas salivales se ve influenciada por la acción exógena de las hormonas sexuales, estrógeno y testosterona (Ferraris y colab. 1986). El objetivo del presente trabajo fue estudiar los mastocitos en forma simultánea en glándulas salivales y ovarios durante el ciclo estrual, para observar las respuestas a la acción de las hormonas sexuales y establecer comparaciones entre ambos órganos. Se usaron 24 ratas Wistar hembras adultas jóvenes. Se determinaron las etapas del ciclo. Se disecaron las glándulas submaxilar (GSM) sublingual (GSL) y ovario, fijándose en formol tamponado y procesado según técnica de rutina. Coloraciones usadas: ATO, ph 3, 8; AB pH 2, 5, PAS. histometria: se realizó en un área de 15,6mm2 a 450 A, con un total de 10 lecturas de cada tejido examinado, áreas periductales y parénquima salival y en ovario: médula, hilio y oviducto. Durante el ciclo estrual se observaron en ambos órganos: 1§ -Variaciones en el comportamiento citoquímico y tamaño celular de los mastocitos especialmente en las etapas del proestro y est ro. 2§ -La GSM en el estro presenta un incremento de mastocitos que es estadísticamente significativo con respecto a las otras etapas. Esto hecho se presenta similar en ovario. Se concluye que durante el ciclo estrual los mastocitos sufren modificaciones citoquímicas y numéricas


Subject(s)
Rats , Animals , Estrus , Histocytochemistry , Mast Cells , Ovary , Sublingual Gland , Submandibular Gland
5.
Rev. Fac. Odontol. (Córdoba) ; 15(1/2): 39-48, ene.-dic. 1987. ilus, tab
Article in Spanish | LILACS | ID: lil-80226

ABSTRACT

En el presente trabajo se cuantifican mastocitos en relación a las modificaciones histológicas de las glándulas salivales de ratas sometidas a la castración y aplicación simultánea de DMBA. Se utilizaron machos jóvenes Wistar, en grupos de cinco animales cada uno. Se sacrificaron a los 12, 23 y 28 días de iniciada la experiencia. Se disecaron las glándulas y se fijan en formol a pH 7 para su estudio morfohistoquímico e histométrico. En la glándula sublingual (GSL) se incrementa el número de mastocitos en todos los plazos estudiados, superando significativamente la población celular de la glándula submaxilar (GSM) a los 12 días de castrados. Al comparar animales sin castrar y castrados se advierte que las diferencias más notables de la población mastocitaria se presentan en (GSL). Con DMBA y castración simultánea se observa que la glándula sublingual sin castrar) condiciona la respuesta tisular atípica de la glándula al efecto del cencerígeno y por ende a la población mastocitaria


Subject(s)
Rats , Animals , 9,10-Dimethyl-1,2-benzanthracene/administration & dosage , Carcinoma , Castration , Mast Cells , Skin Neoplasms/chemically induced , Sublingual Gland , Submandibular Gland
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